Plasmid

Part:BBa_K4047038

Designed by: An Nguyen   Group: iGEM21_MiamiU_OH   (2021-10-06)


pGEM-tal+fbp

Plasmid for overexpressing transaldolase and fructose-1,6-bisphosphatase in Synechococcus elongatus PCC 7942; contains all the components of pAM2991 in addition to our gene

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7968
    Illegal XbaI site found at 2
    Illegal XbaI site found at 2546
    Illegal XbaI site found at 2860
    Illegal PstI site found at 14
    Illegal PstI site found at 2558
    Illegal PstI site found at 5013
    Illegal PstI site found at 5815
    Illegal PstI site found at 9680
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7968
    Illegal NheI site found at 2673
    Illegal NheI site found at 8658
    Illegal PstI site found at 14
    Illegal PstI site found at 2558
    Illegal PstI site found at 5013
    Illegal PstI site found at 5815
    Illegal PstI site found at 9680
    Illegal NotI site found at 515
    Illegal NotI site found at 9933
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7968
    Illegal BglII site found at 9223
    Illegal BglII site found at 9843
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7968
    Illegal XbaI site found at 2
    Illegal XbaI site found at 2546
    Illegal XbaI site found at 2860
    Illegal PstI site found at 14
    Illegal PstI site found at 2558
    Illegal PstI site found at 5013
    Illegal PstI site found at 5815
    Illegal PstI site found at 9680
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7968
    Illegal XbaI site found at 2
    Illegal XbaI site found at 2546
    Illegal XbaI site found at 2860
    Illegal PstI site found at 14
    Illegal PstI site found at 2558
    Illegal PstI site found at 5013
    Illegal PstI site found at 5815
    Illegal PstI site found at 9680
    Illegal NgoMIV site found at 1043
    Illegal NgoMIV site found at 3454
    Illegal NgoMIV site found at 8493
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 198
    Illegal BsaI site found at 2068
    Illegal BsaI site found at 9117
    Illegal BsaI.rc site found at 4839
    Illegal BsaI.rc site found at 5605
    Illegal BsaI.rc site found at 9209
    Illegal SapI site found at 3756


[edit]
Categories
//plasmid
Parameters
None